Coding

Part:BBa_K368002:Design

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-24)


Human Bak + SV40 Polyadenylation site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 839
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 388


Design Notes

induces apoptosis if a cell did not incorporate the gene of interest -> "Jump-Or-Die" system; "Cut'N'survive" system


Source

Human cDNA

References